Chemical composition, aerobic stability and fermentation pattern of tomato pomace and pumpkin waste silage using fibrolytic enzymes and lactic acid bacteria

Document Type : Original Article

Authors

1 Ghaemshahr Branch, Islamic Azad University, Ghaemshahr, Mazandaran, Iran

2 Department of Animal Science, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad kavous, Golestan.Iran

Abstract

Purpose This study aimed to evaluate the effect of different additives on chemical composition, fermentation characteristics, and gas production parameters of tomato pomace and pumpkin waste silages.
Method Treatments were: tomato pomace silage, pumpkin waste silage, tomato pomace and pumpkin waste silage mix (50:50), tomato pomace and pumpkin waste silage mix treated with the fibrinolytic enzyme (E), tomato pomace and pumpkin waste silage mix treated with LAB made inoculants (LMI), and tomato pomace and pumpkin waste silage mix treated with E+ LMI. Representatives of samples were packed manually into laboratory silos and allowed to ensile for 1, 3, 7, 21, 45, and 90 days.
Results The results showed a significant difference between the experimental treatments in chemical composition (p<0.05). The treatment of pumpkin waste showed the lowest amount of dry matter (DM), insoluble fibers in neutral detergent (NDF), and insoluble fibers in acidic detergent (ADF). The value of crude protein (CP) showed a decreasing trend with increasing time after ensiling. The treatment with bacterial and enzymatic additives had a faster drop in pH and a lower final pH compared to other treatments.
Conclusion Compared with the tomato pomace and pumpkin waste silage, treatments E and E + LMI had lower acetic and butyric acid contents. During aerobic exposure, tomato pomace and pumpkin waste had the lowest pH changes in silage. Generally, applying a combination of E and LAB inoculants improved both fermentation quality and aerobic stability of silage.

Highlights

  • The treatment of pumpkin waste showed the lowest amount of dry matter (DM), insoluble fibers in neutral detergent (NDF), and insoluble fibers in acidic detergent (ADF).

 

  • The value of crude protein (CP) showed a decreasing trend with increasing time after ensiling.

 

 

  • The treatment with bacterial and enzymatic additives had a faster drop in pH and a lower final pH compared to other treatments.

 

  • Compared with the tomato pomace and pumpkin waste silage, treatments E and E + LMI had lower acetic and butyric acid contents.

 

 

  • During aerobic exposure, tomato pomace and pumpkin waste had the lowest pH changes in silage.

 

  • Applying a combination of E and LAB inoculants improved both fermentation quality and aerobic stability of silage.

 

Keywords


Adesogan AT, Salawu MB, Deaville E (2002) The effect on voluntary feed intake, in vivo digestibility and nitrogen balance in sheep of feeding grass silage or pea-wheat intercrops differing in pea to wheat ratio and maturity. Anim Feed Sci Technol 96:161-173. https://doi.org/10.1016/S0377-8401(01)00336-4
Adesogan AT, Krueger N, Salawu MB, Dean DB, Staples CR (2004) The influence of treatment with dual purpose bacterial incubation soluble carbohydrates on the fermentation and aerobic stability of Bermuda grass. J Dairy Sci 87: 3407–3416. https://doi.org/10.3168/jds.S0022-0302(04)73476-1
Ajila CM, Brar SK, Verma M, Tyagi RD, Godbout S, Valéro R (2012) Bio-processing of agro-byproducts to animal feed. Crit Rev Biotechno 32(4): 382-400
Aksu T, Baytok E, Akif Karsli M, Muruz H (2006) Effects of formic acid, molasses and inoculant additives on corn silage composition, organic matter digestibility and microbial protein synthesis in sheep. Small Ruminant Res 61: 29-33. https://doi.org/10.1016/j.smallrumres.2004.12.013
AOAC (2000) Official Methods Of Analysis, 17th ed. Association of Official Analytical Chemist, Arlington,VA,USA. ISBN-13 978-093558467-7
Barroso J (2002) A investigação sobre a escola: contributos da Administração Educacional. In: Investigar em Educação. Revista da Sociedade Portuguesa de Ciências da Educação 1(1): 277-325. https://doi.org/10.15687/rec.v10i1.33183
Battaglini M, Costantini F (1978) Byproducts from the tomato industry in diets for growing rabbits. Coniglicoltura 15 (10):19-22. https://www.feedipedia.org/node/7743
Belibasakis NG, Tsirgogianni D (1995) Effects of whole cottonseeds on milk yield, milk composition and blood components of dairy cows in hot weather. Anim Feed Sci Technol 52: 227-235. https://doi.org/10.1016/0377-8401(94)00725-O
Broderick GA, Kang JH (1980) Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J Dairy Sci 63: 64–75. https://doi.org/10.3168/jds.S0022-0302(80)82888-8
Caluya RR (2000) Tomato pomace and rice straw silage for feeding growing cattle. In silage making in the tropics with particular emphasis on smallholders; FAO Plant Production and Protection Paper 161; FAO: Roma, Italy 97–98
Canale A, Valente ME, Ciotti A (1984) Determination of vola-tile carboxylic acids (C1–C5) and lactic acid in aqueous acid ex-tracts of silage by high performance liquid chromatography. J Sci Food Agric  35: 1178–1182. https://doi.org/10.1002/jsfa.2740351106
Colombatto D (2000) Use of enzymes to improve fibre utilizationin ruminants. A biochemical and in vitro rumen degradationassessment. Ph.D. Diss., Univ. of Reading, U.K
Colombatto D, Mould FL, Bhat MK, Phipps RH, Owen E (2004) In vitro evaluation of fibrolytic enzymes as additives for maize (Zea mays) silage. Anim Feed Sci Technol 111: 111-128. https://doi.org/10.1016/j.anifeedsci.2003.08.011
Costa JM, Heuvelink E (2018) The global tomato industry. In Tomatoes; CABI Publishing: Wallingford, UK, pp. 276–313. ISBN 9781780641942
Dean DB, Adesogan AT, Krueger N, Littell RC (2005) Effect of fibrolytic enzymes on the fermentation characteristics, aerobic stability, and digestibility of bermudagrass silage. J Dairy Sci 88: 994-1003. https://doi.org/10.3168/jds.S0022-0302(05)72767-3
Dehghani MR, Weisbjerg MR, Hvelplund T, Kristensen NB (2012) Effect of enzyme addition to forage at ensiling on silage chemical composition and NDF degradation characteristics. Livest Sci 150: 51-58. https://doi.org/10.1016/j.livsci.2012.07.031
Elinge CM, Muhammad A, Atiku FA, Itodo AU, Peni IJ, Sanni OM, Mbongo AN (2012) Premixate  mineral  and  anti-nutrient composition of Pumpkin (Cucurbita pepo L.) seeds extract. J plant 2(5): 146-150. http://dx.doi.org/10.5923/j.plant.20120205.02
Ergül M (1988) Replacement of fishmeal by brewer’s yeast in broiler rations with high levels of cottonseed meal and sunflower seed meal. Landbauforschung Vlkenrode 38 (3): 211-219. https://hdl.handle.net/11454/34111
Eun JS, Beauchemin KA (2007) Enhancing in vitro degradation of alfalfa hay and corn silage using feed enzymes. J Dairy Sci 90: 2839-2851. https://doi.org/10.3168/jds.2006-820
Feedipedia (2011) Tomato pomace: Tomato skins and tomato seeds. https://www.feedipedia.org/node/689
Filya I (2003) The Effect of Lactobacillus buchneri and lactobacillus plantarum on the fermentation, aerobic stability, and ruminal degradability of low dry matter corn and Sorghum silages. J Dairy Sci 86: 3575–3581. https://doi.org/10.3168/jds.S0022-0302(03)73963-0
Filya I, Sucu E, Karabulut A (2006) The effect of Lactobacillus buchneri on the fermentation, aerobic stability and ruminal degradability of maize silage. J Applied Mic 101: 1216-1223. https://doi.org/10.1111/j.1365-2672.2006.03038.x
Fondevila M, Guada JA, Gasa J, Castrillo C (1994) Tomato pomace as a protein supplement for growing lambs. Small Rumin Res 13(2):117-126. https://doi.org/10.1016/0921-4488(94)90086-8
Gallo M, Mlymar R, Rajcakova L (2001) The effect of the combination of biological and biological-enzymatic additive wit sodium benzoate upon the fermentation process in red clover silages. In: The Xth International Symposium Forage Conservation, Brno: MZLU Brno, pp. 100-101
Gallo J, Fernye C, Orosz S, Katona K, Szemethy L (2017) Tomato pomace silage as apotential new supplementary food for game species. Agri and food sci 26: 80-90. https://doi.org/10.23986/afsci.59665
Gasa J, Castrillo C, Baucells MD, Guada JA (1989) By-products from the canning industry as feedstuff for ruminants: Digestibility and its prediction from chemical composition and laboratory bioassays. Anim Feed Sci Technol 25 (1-2): 67-77. https://doi.org/10.1016/0377-8401(89)90108-9
Gordon FJ, Dawson LER, Ferris CP, Steen RWJ, Kilpatrick DJ (1999) The influence of wilting and forage additive type on the energy utilisation of grass silage by growing cattle. Anim Feed Sci Technol 79(1-2):15-27. https://doi.org/10.1016/S0377-8401(99)00013-9
Haghparvar R, Shojaian K, Rowghani E, Parsaei S, Yousef Ellahi M (2012) The effects of Lactobacillus plantarum on chemical composition, rumen degradability, in vitro gas production and energy content of whole-plant corn ensiled at different stages of maturity. Iran J Veter Res 13(38): 8-15
Hassanat F, Mustafa AF, Seguin P (2007) Effects of inoculation on ensiling characteristics, chemical composition and aerobic stability of regular and brown midrib millet silages. J Anim Sci 139: 125-140. https://doi.org/10.1016/j.anifeedsci.2007.01.005
Higginbotham GE, DePeters EJ, Mueller SC (1996)  Effect   of  propionic acid producing bacteria on corn silage fermentation. Prof Anim Sci 12: 176-180. https://doi.org/10.15232/S1080-7446(15)32514-6
Islam M, Enishi O, Purnomoadi A, Higuchi K, Takusari N, Terada F (2001) Energy and protein utilization by goats fed Italian ryegrass silage treated with molasses, urea, cellulase or cellulase + lactic acid bacteria. Small Rminant Research 42: 49-60. https://doi.org/10.1016/S0921-4488(01)00235-8
Jaakkola S, Huhtanen P, Hissa K (1991) The effect of cell wall degrading enzymes or formic acid fermentation quality on digestion of grass silage by cattle. Grass Forage Sci 46 (1):75–87. https://doi.org/10.1111/j.1365-2494.1991.tb02209.x
Jatkauskas J, Vrotniakiene V (2004) Improvement of grass silage quality by inoculant with lactic bacteria and enzymes. Veterinarija ir Zootechnika 28: 79-82
Keady TWJ, Steen RWJ, Kilpatrick. DJ, Mayne CS (1994) Effects of inoculant treatment on silage fermentation, digestibility and intake by growing cattle. Grass Forage Sci 49: 284-294. https://doi.org/10.1111/j.1365-2494.1994.tb02003.x
Kizilsimsek M, Schmidt RJ, Kung L (2007) Effects of a mixture of lactic Acid bacteria applied as a freeze-dried or fresh culture on the fermentation of alfalfa silage. J Dairy Sci 90: 5698-5705. https://doi.org/10.3168/jds.2007-0448
Koc F, Coskuntuna L, Ozduven L (2008) The effect of bacteria + enzyme mixture silage inoculant on the fermentation characteristics, cell wall contents and aerobic stabilities of maize silage. Pakistan J Anim Sci 7: 222-226. https://dx.doi.org/10.3923/pjn.2008.222.226
Kubota C, De Gelder A, Peet MM (2018) Greenhouse tomato production. In tomatoes; CABI Publishing: Wallingford, UK, pp. 276–313. ISBN 9781780641942
Kung L, Tung RS, Maciorowski KG, Buffum K, Knutsen K, Aimutis WR (1991) Effects of plant cell-wall-degrading enzymes and lactic acid bacteria on silage fermentation and composition. J Dairy Sci 74: 4284-4296. https://doi.org/10.3168/jds.S0022-0302(91)78623-2
Kung JL, Ranjit NK (2001) The effect of Lactobacillus buchneri and other additives on the fermentation and aerobic stability of Barley silage. J Dairy Sci 84: 1149–1155. https://doi.org/10.3168/jds.s0022-0302(01)74575-4
Kung LM, Shaver R (2001) Interpretation and use of silage fermentation analysis reports. Focus Forage, 3:1-5
Kung Jr L, Myers CL, Neylon JM, Taylor CC, Mills JA, Whiter AG (2004) The effects of buffered propionic acid-based additives alone or combined with microbial inoculation on the fermentation of the high moisture corn and whole-crop barley. J Dairy Sci 87: 1310-1316. https://doi.org/10.3168/jds.S0022-0302(04)73280-4
Lynch JP, Jin L, Lara EC, Baah J, Beauchemin KA (2014) The effect of exogenous fibrolytic enzymes and a ferulic acid esterase-producing inoculant on the fiber degradability, chemical composition and conservation characteristics of alfalfa silage. Anim Feed Sci Technol 193: 21-31. https://doi.org/10.1016/j.anifeedsci.2014.03.013
Makkar HPS (2005) In vitro gas methods for evaluation of feeds containing phytochemicals. Anim Feed Sci 123-124: 291-302. https://doi.org/10.1016/j.anifeedsci.2005.06.003
McAllister TA, Selinger LB, McMahon LR, Bae HD, Lysyk TJ, Oosting SJ, Cheng KJ (1995) Intake, digestibility and aerobic stability of barley silage inoculated with mixtures of barley silage of lactobacillus plantarum and Entrococcuse faecium. Can J Anim Sci 75:425-432. https://doi.org/10.4141/cjas95-062
McAllister T, Hristov  AN, Beauchemin K, Rode L, Cheng KJ (2001) Enzymes in ruminant diets. Enzymes in Farm Animal Nutrition 273-298. https://doi.org/10.1079/9780851993935.0273
McDonald P, Henderson AR, Heron SJE (1991) The biochemistry of silage (2nded), Chalcombe, U.K. 184p. https://doi.org/10.1017/S0014479700023115
Mendoza Octavio Loera-Corral, Fernando X Plata-Pérez, Pedro A Hernández-García, Mónica Ramírez-Mella (2014) Considerations on the use of Exogenous Fibrolytic enzymes to improve forage utilization. The Sci World J 1-9. https://doi.org/10.1155/2014/247437
Menke KH, Raab L, Salewski A, Steingass H, Fritz D, Schneider W (1979) The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro. J Agric Sci 93: 217-222. https://doi.org/10.1017/S0021859600086305
Muck RE, Bolsen KK (1991) Silage preservation and silage additive products. Page 105 in Field Guide for Hay and Silage Management in North America. Bolsen K K, Baylor JE, McCullough ME, eds. Natl. Feed Ingred. Assoc., West Des Moines, IA
Nadeau EMG, Buxton DR, Russell JR, Allison MJ, Young JW (2000) Enzyme, bacterial inoculant, and formic acid effects on silage composition of orchardgrass and alfalfa. J Dairy Sci 83: 1487-1502. https://doi.org/10.3168/jds.s0022-0302(00)75021-1
Ohyama Y, Morichi T, Masahi S (1975) The effect of inoculation with Lactobacillus plantarum and the addition of glucose at ensiling on the quality of aerated silages. J Sci Food Agri 26: 1001-1008. https://doi.org/10.1002/jsfa.2740260717
Orskov ER, McDonald I (1979) The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J Agri Sci Camb 92: 499-503. https://doi.org/10.1017/S0021859600063048
Oude Elferink SJ, Krooneman J, Gottschal JC, Spoelstra SF, Faber F, Driehuis F (2001) Anaerobic conversion of lactic acid to acetic acid and 1, 2-propanediol by Lactobacillus buchneri. Appl Environ Microbiol 67:125–132. https://doi.org/10.1128/AEM.67.1.125-132.2001
Pitt RE, Liu Y, Muck RE (1991) Stimulation of the effect of additives on aerobic stability of alfalfa and corn silages. T ASAE 34:1633–1641. https://doi.org/10.13031/2013.31781
Rauramaa A, Setala J, Moisio T, Heikkila T, Lampila M (1987) The effect of inoculants and cellulase on the fermentation and microbiological composition of grass silages. J Agric Finl 59:371-377. https://www.sciencedirect.com
Rodrigues MAM, Cone JW, Sequeir CA, Mascarenhas Ferreira A (2001) Effect of the addition of cell wall degrading enzymes on fermentation kinetics of perennial ryegrass silage. J Agric Sci 136: 443-449. https://doi.org/10.1017/S0021859601008954
Rotz CA, Muck RE (1994) Changes in forage quality during harvest and storage in forage quality, evaluation and utilization. G. Fahey, ed. Am. Soc. Agron Crop Sci Soc Am., Madison, WI. Pp 828–868. https://doi.org/10.2134/1994.foragequality.c20
Sadeghi Khorvash K, Ghorbani M, Forouzmand GR, Boroumand MA, Hashem zadeh Cigari F (2012) Effects of homo-fermentative bacterial inoculants on fermentation characteristics and nutritive value of low dry matter corn silage. Iran J Veterinary Res Shiraz University 13(4): 303-309. https://dx.doi.org/10.22099/ijvr.2012.610
Stokes MR (1992) Effects of an enzyme mixture, an inoculants, and their interaction on silage fermentation and dairy production. J Dairy Science 75:765. https://doi.org/10.3168/jds.S0022-0302(92)77814-X
Stokes MR, Chen J (1994) Effects of an enzyme-inoculant mixture on the course of fermentation of corn silage. J Dairy Sci 77: 3401-3409. https://doi.org/10.3168/jds.S0022-0302(94)77282-9
Tang SX, Tayo GO, Tan ZL, Sun ZH, Shen LX, Zhou CS, Xiao WJ, Ren GP, Han XF, Shen SB (2000) Effects of yeast culture and fibrolytic enzyme supplementation on in vitro fermentation characteristics of low-quality cereal straws. J Anim Sci 86(5): 1164–1172. https://doi.org/10.2527/jas.2007-0438
Theodorou MK, Williams BA, Dhanoa MS, McAllan AB, France J (1994) A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Anim Feed Sci Technol 48:185–197. https://doi.org/10.1016/0377-8401(94)90171-6
Tjardes KE, Buskirk DD, Allen MS, Amest NK, Bourquin LD, Rust SR (2000) Brown midrib-3 corn silage improves digestion but not performance of growing beef steers. J Anim Sci 78: 2957–2965. https://doi.org/10.2527/2000.78112957x
Ulger I, Kaliber M, Ayahan T, Küçük O (2018) Chemical composition, organic matter digestibility and energy content of apple pomace silage and its combination with corn plant, sugar beet pulp and pumpkin pulp. South African J Anim Sci 48: 497–503
Valizadeh R, Naserian AA, Ajdari fard A (2007) The biochemistry of silage. Ferdowsi Univercity Press. 414PP. https://doi.org/10.4314/sajas.v48i3.10
Van Soest PJ, Robertson JB, Lewis BA (2000) Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 74: 3583–3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
Whiter AG, Kung Jr L (2001) The effect of a dry or liquid application of Lactobacillus plantarum MTD1 on the fermentation of Alfalfa silage. J Dairy sci 84: 2195–2202. https://doi.org/10.3168/jds.s0022-0302(01)74666-8
World Processing Tomato Council (WPTC) (2019) World production estimate as of 12 February available  online: https://www.wptc.to/pdf/releases/.  WPTC world production estimateas of   12 February 2019
Xing L, Chen LT, Han LJ (2008) The effect of on inoculants and enzymes on fermentation and nutritive value of sorghum straw silages. Bioresour Technol 100: 488-491. https://doi.org/10.1016/j.biortech.2008.06.017
Yitbarek M, Tamir B (2013) Silage additives: Review. Open J Applied Sci 258-274. http://dx.doi.org/10.4236/ojapps.2014.45026
Zobell DR, Weidmeier RD, Olson KC, Teracher RH (2000) The effect of an exogenous enzyme treatment on production and carcass characteristics of growing and finishing steers. Anim Feed Sci and Technol 87: 279283. https://doi.org/10.1016/S0377-8401(00)00202-9.