Conversion of food waste via two-stage fermentation to controllable chicken Feed Nutrients by local isolated microorganism

Document Type : Original Article


1 Bioprocess Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800, Pulau Pinang

2 E-Idaman Sdn. Bhd., Wisma Idaman, No. 163 & 164,Jalan BSG 4 Bandar Stargate,Lebuhraya Sultanah Bahiyah,05400 Alor Setar, Kedah.

3 Solid Waste Management Cluster, Science and Engineering Research Centre, Engineering Campus, Universiti Sains Malaysia, 14300 Nibong Tebal, Seberang Perai Selatan, Penang, Malaysia.


Purpose Food waste can be referred to as edible food materials that can create problems to the environment if it is not disposed properly. Therefore, the purpose of this study is to produce chicken feed from food waste via two-stage fermentation.
Methods Food waste was converted with combination of fungi A (isolated from degraded onion) and yeast B (isolated from local fermented fruit - Durian). Four batches of food waste were obtained from different sources. Food waste was fermented with fungi A and yeast B in bioreactor for 5 days with 80% moisture content. Fermented food waste was then dried in the oven.
Results Glucose and carbohydrate contents were investigated during fermentation process and it was found that carbohydrate content decreased from 0.1857 g/g before fermentation to 0.1305 g/g after fermentation. Glucose content was found to increase at the first 48 hours and then dropped from 48th hour until the fermentation was done. To get consistent with the results of the last product, the process control elements such as C:N ratio, pH, aeration, agitation, temperature, antiseptic technique and sterilization of food waste were controlled. The standard of the fermented food waste was compared with the standard of chicken feed used in Malaysia. The crude protein content, crude fat content, crude fiber content, total ash content and total energy content of chicken feed met the requirement set by Malaysia standard (MS 20:2008) of chicken feed.
Conclusion Thus, it is shown that food waste has a massive potential in the production of chicken feed.



Abd Razak S (2017) Household food waste prevention in Malaysia: An issue processes model perspective. Graduate theses and dissertations.  Retrieved from (Accessed on 28 September 2018).
Amal AA, Mohamed NAEG, Eman HA (2018) Citric acid fermentation by Aspergillus niger. JIPBS5(4): 20-37.
AOAC An Official Methods of Analysis of AOAC International (2012) 19th Ed.,   AOAC International, Gaithersburg, MD, USA.
Applegate T, Angel R (2008) Phosphorus requirements for poultry. A key ingredient in livestock and poultry nutrient management. USDA, Washington DC.
Bandeswaran C (2016) Nutrient composition of food wastes and its utilization strategy as livestock feed to reduce environment pollution. IJONAS 3(5): 183-187.
Banhazi TM, Babinszky L, Halas V, Tscharke M (2012) Precision livestock farming: Precision feeding technologies and sustainable livestock production. Int. J. Agric. Biol. Eng 5(4): 54-61. 10.3965/j.ijabe.20120504.006
Buschke N, Schafer R, Becker J, Wittmann C (2013) Metabolic engineering of industrial platform microorganisms for biorefinery applications-optimization of substrate spectrum and process robustness by rational and evolutive strategies. Bioresour Technol  135: 544-554.
Chen H, Jiang W, Yang Y, Yang Y, Man X (2017) State of the art on food waste research: a bibliometrics study from 1997 to 2014. J. Clean. Prod 140: 840-846. jclepro.2015.11.085
Chiba LI (2014) Diet formulation and common feed ingredients in animal nutrition handbook, section 12: Poulty Nutrition and Feeding. pp. 410-425.
Chintagunta AD, Jacob S, Banerjee R (2016) Intergrated bioethanol and biomanure production from potato waste. Waste Manag 49: 320-325. https://doi.10.1016/j.wasman. 2015.08.010
Coote N, Kirsop BH (1976) Factors responsible for the decrease in pH during beer fermentation. J. Inst. Brew 82: 149-153.
Couto SR, Sanroman MA (2006) Application of solid-state fermentation to food industry - A review. J. Food Eng 76: 291-302.
DVS (Department of Veterinary Services) (2012) Downloaded from c74bc4b8-907c-407d-8426- b37cc7ce2b81 on 28/09/2018.
EPA (Environmental Protection Agency) (2003) Test methods, U.S EPA New England Region 1 Library, Boston, MA USA.
Freigassner M, Pichler H, Glieder A (2009) Tuning microbial hosts for membrane protein production. Microb Cell Fact 8: 69. https://doi.10.1186/1475-2859-8-69
Ghasem DN (2007) Chapter 12- Production of Citric Acid. Biochemical Engineering and Biotechnology, pp. 280-286.
Gupta A, Gupta V, Modi D, Yadava L (2008) Production and characterization of α-amylase from Aspergillus niger. J. Biotechnol7(3): 551-556. https://doi.10.3923/biotech. 2008.551.556
Gustavsson J, Cederberg C, Sonesson U, van Otterdijk R, Meybeck A (2011) Global food losses and food waste: Extent, Causes and Prevention. FAO.
Han SK, Shin HS (2004) Biohydrogen production by anaerobic fermentation of food waste. Int. J. Hydrog. Energy 29(6): 569 – 577.
Hesse SJA, Ruijter GJG, Dijkema C, Visser J (2002) Intracellular pH homeostasis in the filamentous fungus Aspergillus niger. Eur J Biochem 269: 3485–3494. https://doi.10.1046/j.1432-1033.2002.03042.x
Hirschmann R (2019) Poultry consumption per capita in Malaysia from 2009 to 2025 (in kilograms). Retrieved from on 23 August 2019)
IS (Indian Standard) 3025 (Part 32) (1988, Reaffirmed 2007): Method of sampling and test (physical and chemical) for water and wastewater, Part 32: Chloride (First Revision).
Khetarpaul N, Chauhan BM (1989) Effect of fermentation on protein, fat, minerals and thiamine content of pearl millet. Plant Food Hum Nuty 39: 169-177.
Kim M, Chowdhury M, Nakhla G, Keleman M (2015) Characterization of typical household food wastes from disposers: fractionation of constituents and implications for resource recovery at wastewater treatment. Bioresour. Technol183: 61-69. 2015.02.034
Liang J, Han BZ, Nout MJR, Hamer RJ (2008) Effects of soaking, germination and fermentation on phytic acid, total and in vitro soluble zinc in brown rice. Food Chem 110: 821–828. https://doi.10.1016/j.foodchem.2008.02.064
Lim WJ, Chin NL, Yusof AY, Yahya A, Tee TP (2016) Food waste handling in Malaysia and comparison with other Asian countries. IRRJ 23 (Suppl): S1-S6.
Ma H, Wang Q, Qian D, Gong L, Zhang W (2009) The utilization of acid-tolerant bacteria on 11 ethanol production from kitchen garbage. RE 34(6): 1466–1470. https://doi.10.1016/j.renene.2008.10.020
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing  sugar. Anal. Chem31(3): 426-428.
Ministry of Agriculture (2005) Livestock products statistic. Retrieved from. (Accesed on 3 November 2018).
Mountney GJ, Gould WA (1988) Practical food microbiology and technology. AVI Books, Van Nostrand Reinhold Company, New York, USA.
Nesheim MC, Leach RM, Zegler TR, Serafin JA (1964) Interrelationships between dietary levels of sodium, chlorine and potassium. J. Nutr 84: 361–375. https://doi. 10.1093/jn/84.4.361
Obadina AO, Akinola OJ, Shittu TA, Bakare HA (2013) Effect of natural fermentation on the chemical and nutritional 
composition of fermented soymilk nono. Nigerian Food Journal 31(2): 91-97.
Penz AM Jr (1988) Acid-base balance and their relation with problems in broiler production. pp. 115–130.
Pond WG, Church DC, Pond KR John Wiley, Sons, Inc; Canada (1995) Basic animal nutrition and feeding.
Raamsdonk LM, Diderich JA, Kuiper A, Van Gaalen M, Kruckeberg AL, Berden JA, Van Dam K  (2001) Co-consumption of sugars or ethanol and glucose in a Saccharomyces cerevisiae strain deleted in the HXK2 gene. Yeast 18: 1023-1033.
ReFED (Rethink Food Waste through Economics and Data) (2016) A roadmap to reduce U.S. food waste by 20 percent. Retrieved from downloads/Executive-Summary.pdf (Accessed on 26 September 2018)
Sadasivam S, Manickam A (1996) Biochemical methods: New Age International.
Sheikh AH, Tariq M, Afsheen A (2012) Reduction of chemical and biochemical oxygen demand after treatment of pharmaceutical effluents. Pak. J. Pharm25 (1, 2): 9-13.
Stuart T (2009) Waste: Uncovering the global food scandal (first ed.), W.W. Norton, Co, New York.
Teresa G, Vito P, Giorgio C, Giuseppa DB, Nicola C, Giacomo D (2018) Production of single cell protein (SCP) from food and agricultural waste by using Saccharomyces cerevisiae. Natural Product Research 32(6): 648-653.
Themelis NJ (2010) Chlorine sources, sinks, and impacts in WTE power plants. Paper presented at the 18th Annual North American Waste-to-Energy Conference.
Thongdumyu P, Intrasungkha N, Sompong O (2014) Optimization of ethanol production from food waste hydrolysate by co-culture of Zymomonas mobilis and Candida shehatae under non-sterile condition. Afr. J. Biotechnol 13(7): 866. AJB2013.13335
Tommaso G (2011) Effluents from the food industry. Woodhead Publishing Series in Food Science, Technology and Nutrition, pp. 606-622.
Umeh GN, Udo BI (2002) Profitability of poultry production among school leavers in Anambra State, Nigeria. Niger. J. Anim. Prod 29(1): 76-80.
Wahyono MD, Utami MMD (2017) A review of the poultry meat production industry for food safety in Indonesia. The 2nd International Joint Conference on Science and (IJCST) 2017. IOP Conf. Series: J. Phys: Conf. Series 953 (2018). https://doi.10.1088/1742-6596/953/1/012125
Westendorf ML (2000) Food waste as animal feed: an introduction. M.L. Westendorf (Ed.), Food Waste to Animal Feed, Iowa State University Press, Ames, IA, pp. 91-111.
Xue L, Liu G, Parfitt J, Liu X, Van Herpen, E, Stenmarck Å, Cheng S (2017) Missing food, missing data? A critical review of global food losses and food waste Data. Environ. Sci. Technol 51(12): 6618–6633. 10.1021/acs.est.7b00401
Zheng XD, John DT, Westendorf ML (2018) Food waste for livestock feeding: Feasibility, safety, and sustainability implications. Glob Food Secur 17: 154 – 161. https://doi. org/10.1016/j.gfs.2017.12.003